‘哈伯’南天竹组织培养和快速繁殖

为建立‘哈伯’南天竹组织培养和种苗繁育技术体系,以半木质化带芽茎段为外植体材料开展植株再生研究。通过观察对比试验法、L₉(3⁴)正交试验设计完全随机法、极差分析、显著性检验、LSD多重比较,探讨了‘哈伯’南天竹组培的最适培养基配方。试验结果表明：最佳诱导培养基为MS + 6-BA 2.0 mg/L + IBA 0.1 mg/L +蔗糖30 g/L,诱导萌动率71.77%,成活率85.51%;最佳增殖培养基为WPM +6-BA 1.5 mg/L + IBA 0.01 mg/L + 蔗糖30 g/L,增殖系数6.3;最佳生根培养基为1/2 MS+ IBA 0.5 mg/L + NAA 1.0 mg/L + 蔗糖20 g/L + AC 0.2 g/L,生根率97.63%;试管苗移入泥炭土:珍珠岩=3:2(V/V)混合基质中,移栽成活率96.67%。该试验建立了高效稳定的组培快繁技术体系,得到的组培苗后代能够稳定的保持母本优良性状,为工厂化育苗提供了技术支撑。     

Screening and identification of soybean varieties with high phosphorus efficiency at seedling stage

In order to simplify the workload and find a reliable method for screening soybeans with different phosphorus(P)efficiencies,47 soybean varieties were screened from 90 varieties according to yield under normal phosphorus,and 10 indicators including root phenotype,phosphorus utilization efficiency at the seedling stage and yield were measured.Through single-index cluster analysis,the performance value and relative value of the abovementioned indexes under low-phosphorus conditions were analyzed,and then,the combined indexes were analyzed by principal components method.The membership function method and the cluster analysis method was used to calculate and analyze the comprehensive score value.Three indexes of root P utilization efficiency(X₁),relative value of root P utilization efficiency(X₂),and root volume(X₃)under low P at the seedling stage were selected as the most consistent with the yield screening results under low P condition,and the mathematical model of the comprehensive score value was obtained:D=1.218X₁t 0.320X₂t 0.007X₃-0.664(P=0.000,R²=1.000).The comprehensive score can screen and identify the P efficiency of soybeans with fewer indexes in the early growth stages,which provides a more rapid and reliable mathematical model for screening and identifying a larger number of germplasm resources for P efficiency.     

株行距及穴苗数的配置对寒地水稻产量和品质的影响

为明确垦稻26和龙粳31高产优质栽培的株行距和穴苗数处理,采取裂区设计进行试验研究。结果表明,最优群体因品种而异,垦稻26在株距10cm、行距30cm、5苗/穴产量最佳,龙粳31则以株距13.3cm、行距27cm、9苗/穴产量最佳;垦稻26在5苗/穴、行株距30cm×10cm处理,有利于提高稻米的碾磨品质,不利于改善稻米的外观品质,营养品质较低,利于改善稻米的食味品质,且食味评分值高达86.5,显著高于最低处理;龙粳31在5苗/穴、行株距27cm×13.3cm处理,碾磨品质较高,不利于营养品质和外观品质的改善,食味品质得到明显改善,食味评分值高达86.0,与最低处理差异达显著水平。因此,高产优质栽培要因品种选择适宜的穴苗数和株行距,是实现水稻高产优质最为快捷、最为经济有效的措施。     

EDTA对铅胁迫下萝卜芽苗菜生长的影响

以萝卜芽苗菜为材料,在其生长期外施不同浓度EDTA,初步探讨了EDTA对铅胁迫下萝卜芽苗菜部分生理生化特性的影响.结果表明:在300 mg/L铅胁迫浓度下萝卜芽苗菜的膜透性明显增大,过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、可溶性蛋白含量明显下降.随着外施EDTA浓度的增加,萝卜芽苗菜的膜透性先下降后升高,过氧化物酶(POD)活性、超氧化物歧化酶(SOD)活性、可溶性蛋白含量先升后降.就供试材料而言,在一定浓度范围内的EDTA对铅胁迫萝卜芽苗菜的伤害效应有缓解作用,其中以0.75 μmol/L EDTA处理效果最为明显.     

多效疏导素对水稻秧苗素质及产量影响的研究

研究了多效疏导素水溶液对水稻秧苗素质和产量的影响。结果表明,采用适宜的浓度穴10‰雪多效疏导素水溶液对水稻秧苗素质有显著提高,可提高水稻产量达8.0%;过高的浓度,则会明显抑制水稻秧苗生长,造成减产。     

苹果组培芽露地嫁接技术研究

提高苹果组培芽露地嫁接成活率的关键技术措施,主要有三个方面:培育壮芽,嫁接前对组培芽进行3至5天通风锻炼,增强其对外部环境条件的适应性;正确选择砧木及嫁接部位和方法,尽可能缩小砧穗间形态结构及木质化程度的差异,人为创造适宜的环境条件,搭棚遮荫避免阳光直射,适时灌水增加土壤及空气湿度,滴注激素促进接口愈合.本试验组培芽露地嫁接成活率达到93.3%,枝条最大月生长量为38厘米.     

APPLICATION AND EFFECTS OF RARE-EARTH ON SEEDLINGS

In order to improve the quality of seedlings and economic benefits,rare--earth experiments were carried out on eleven seedling-age-types of four silvicultral treespecies.Some indexes such as the increment,biomass and chlorophyl content weredetermined.The results showed that rare-earth can improve the growth in height anddiameter,increase the bioprductivity and increase the mean benefit by887766.29 yuan/hm~2.It is an effective method to improve the quality of seedlings for the characters such as lowcost and easy grasping.And the method can be widely used in seedling.     

拖拉机集材对迹地土壤条件及苗木生长影响的研究(Ⅱ)——拖拉机对集材道苗木生长影响的灰色动态分析

履带式拖拉机和轮式拖拉机由于其走行机构的不同,其集材道土壤压实也存在差异,特别衡量土壤压实的两个重要指标——土壤硬度和孔隙度——两种机型不一致。通过对调查测定数据进行计算处理以及用灰色系统理论进行动态分析,结果表明:与轮式拖拉机相比,履带式拖拉机对苗木生长是有利的。     

多花黑麦草播期试验(1987～1988年)

本试验研究了7个不同播期对多花黑麦草出苗、生产性能的影响.结果表明:在江西清江种草养畜试验站从8月中旬至11月中旬均可播种多花黑麦草,尤以9月中旬至10月中旬更为适宜.     

4种多年生禾草苗期抗旱性的比较研究

采用盆栽育苗法,在4种多年生禾本科牧草苗期连续干旱胁迫下测定植株的叶片相对含水量、质膜相对透 性、游离脯氨酸含量、丙二醛含量4个抗旱生理指标,以综合评价4种多年生禾草的抗旱性能。研究结果表明:供试材料 在连续干旱胁迫下,叶片相对含水量呈下降趋势,质膜相对透性、游离脯氨酸含量及丙二醛含量都有所增加。综合分析 结果,4种多年生禾草的苗期抗旱性按照从强到弱的顺序依次为无芒雀麦>苇状羊茅≥老芒麦>披碱草。